Corrigendum: Variation-preserving normalization unveils blind spots in gene expression profiling
نویسندگان
چکیده
This corrects the article DOI: 10.1038/srep42460.
منابع مشابه
Variation-preserving normalization unveils blind spots in gene expression profiling
RNA-Seq and gene expression microarrays provide comprehensive profiles of gene activity, but lack of reproducibility has hindered their application. A key challenge in the data analysis is the normalization of gene expression levels, which is currently performed following an implicit assumption that most genes are not differentially expressed. Here, we present a mathematical approach to normali...
متن کاملA novel normalization approach unveils blind spots in gene expression profiling
RNA-Seq and gene expression microarrays provide comprehensive profiles of gene activity, by measuring the concentration of tens of thousands of mRNA molecules in single assays [1, 2, 3, 4, 5]. However, lack of accuracy and reproducibility [6, 7, 8, 9] have hindered the application of these high-throughput technologies [10, 11]. A key challenge in the data analysis is the normalization of gene e...
متن کاملارزیابی راندمان تعدادی از ژنهای مرجع در آلوروپوس تحت شرایط تنش شوری
Application of wild type crops or wild relatives’ cultivars that are halophyte in plant breeding program would provide better results in order to develop cultivars that are resistant toward drought as well as salinity. Recently, Aeluropus littoralis has attracted the attention of researcher in order to identify novel genes and their regulatory elements that are involved in salinity stress....
متن کاملSemiquantitative RT-PCR analysis to assess the expression levels of iNOS in chicken macrophages
Abstract: iNOS is inducible by a variety of factors related to inflammation and referred to as inducible NOS(iNOS). It is regulated at the level of gene expression; once expressed, it produces NO at a high rate. iNOS gene-expression profiling is an important tool in understanding molecular markers of the responses of cells and tissues to external factors. In this article a semiquantitative reve...
متن کاملHow to Choose a Normalization Strategy for miRNA Quantitative Real-Time (qPCR) Arrays
Low-density arrays for quantitative real-time PCR (qPCR) are increasingly being used as an experimental technique for miRNA expression profiling. As with gene expression profiling using microarrays, data from such experiments needs effective analysis methods to produce reliable and high-quality results. In the pre-processing of the data, one crucial analysis step is normalization, which aims to...
متن کامل